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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/01/2014 |
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Data da última atualização: |
31/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HUNGRIA, M.; KASCHUK, G. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO; GLACIELA KASCHUK, UNIPAR. |
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Título: |
Regulation of N2 fixation and NO3-/NH4+ assimilation in nodulated and N-fertilized Phaseolus vulgaris L. exposed to high temperature stress. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Environmental and Experimental Botany, v. 98, p. 32-39, Feb. 2014. |
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DOI: |
http://dx.doi.org/10.1016/j.envexpbot.2013.10.010 |
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Idioma: |
Inglês |
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Conteúdo: |
Legumes need large amounts of N to grow satisfactorily. Under low NO3− availability in the soil, many legumes meet their N requirements by N2 fixation in association with rhizobia. Both NO3− uptake and N2 fixation decrease as temperature exceeds optimal growth conditions, but the mechanisms of regulation of N2 fixation and NO3−/NH4+ assimilation under high temperature stress are not completely understood. We describe an experiment in which physiological mechanisms regulating N metabolism of common bean (Phaseolus vulgaris L.) are investigated in plants submitted to daily maximum temperatures of 28, 34 and 39 °C. Common bean was grown in symbiosis with each of six rhizobial strains—belonging to four different species and varying in N2 fixation effectiveness—or fertilized with NO3− until flowering. Harvest measurements included the activities of shoot, stem and root NO3− reductase (NR), nodule glutamine synthetase (GS), NADH-dependent glutamate synthase (GOGAT), nitrogenase, phosphoenol pyruvate carboxylase (PEPcase), N-export rates by nodules and concentration of N compounds in the xylem sap. Higher temperatures inhibited N2 fixation resulting in lower proportion of ureide-N in nodules and xylem sap of nodulated plants in relation to amide-N and α-amino-N. Higher temperatures consistently reduced the activity of NR in leaves of N-fertilized plants. Higher temperatures also decreased N exported from nodules and activities of nitrogenase, GS, GOGAT and PEPcase. The rate of decreases varied in plants with different strains. Furthermore, the activities of GS and GOGAT were more strongly affected by high temperatures than the activity of nitrogenase. There was a remarkable increase in the concentration of NH4+-N and ureide-N in the nodules when GS and GOGAT activities decreased. Therefore, the results provide evidence that N2 fixation in common bean submitted to heat stress is limited by NH4+ assimilation via GS-GOGAT rather than by decreased activity of nitrogenase. Rhizobial effectiveness determined the degree of down-regulation of GS-GOGAT activity in nodule tissues. MenosLegumes need large amounts of N to grow satisfactorily. Under low NO3− availability in the soil, many legumes meet their N requirements by N2 fixation in association with rhizobia. Both NO3− uptake and N2 fixation decrease as temperature exceeds optimal growth conditions, but the mechanisms of regulation of N2 fixation and NO3−/NH4+ assimilation under high temperature stress are not completely understood. We describe an experiment in which physiological mechanisms regulating N metabolism of common bean (Phaseolus vulgaris L.) are investigated in plants submitted to daily maximum temperatures of 28, 34 and 39 °C. Common bean was grown in symbiosis with each of six rhizobial strains—belonging to four different species and varying in N2 fixation effectiveness—or fertilized with NO3− until flowering. Harvest measurements included the activities of shoot, stem and root NO3− reductase (NR), nodule glutamine synthetase (GS), NADH-dependent glutamate synthase (GOGAT), nitrogenase, phosphoenol pyruvate carboxylase (PEPcase), N-export rates by nodules and concentration of N compounds in the xylem sap. Higher temperatures inhibited N2 fixation resulting in lower proportion of ureide-N in nodules and xylem sap of nodulated plants in relation to amide-N and α-amino-N. Higher temperatures consistently reduced the activity of NR in leaves of N-fertilized plants. Higher temperatures also decreased N exported from nodules and activities of nitrogenase, GS, ... Mostrar Tudo |
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Palavras-Chave: |
Fixação biológica de nitrogênio. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02719naa a2200157 a 4500
001 1975792
005 2017-07-31
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.envexpbot.2013.10.010$2DOI
100 1 $aHUNGRIA, M.
245 $aRegulation of N2 fixation and NO3-/NH4+ assimilation in nodulated and N-fertilized Phaseolus vulgaris L. exposed to high temperature stress.
260 $c2014
520 $aLegumes need large amounts of N to grow satisfactorily. Under low NO3− availability in the soil, many legumes meet their N requirements by N2 fixation in association with rhizobia. Both NO3− uptake and N2 fixation decrease as temperature exceeds optimal growth conditions, but the mechanisms of regulation of N2 fixation and NO3−/NH4+ assimilation under high temperature stress are not completely understood. We describe an experiment in which physiological mechanisms regulating N metabolism of common bean (Phaseolus vulgaris L.) are investigated in plants submitted to daily maximum temperatures of 28, 34 and 39 °C. Common bean was grown in symbiosis with each of six rhizobial strains—belonging to four different species and varying in N2 fixation effectiveness—or fertilized with NO3− until flowering. Harvest measurements included the activities of shoot, stem and root NO3− reductase (NR), nodule glutamine synthetase (GS), NADH-dependent glutamate synthase (GOGAT), nitrogenase, phosphoenol pyruvate carboxylase (PEPcase), N-export rates by nodules and concentration of N compounds in the xylem sap. Higher temperatures inhibited N2 fixation resulting in lower proportion of ureide-N in nodules and xylem sap of nodulated plants in relation to amide-N and α-amino-N. Higher temperatures consistently reduced the activity of NR in leaves of N-fertilized plants. Higher temperatures also decreased N exported from nodules and activities of nitrogenase, GS, GOGAT and PEPcase. The rate of decreases varied in plants with different strains. Furthermore, the activities of GS and GOGAT were more strongly affected by high temperatures than the activity of nitrogenase. There was a remarkable increase in the concentration of NH4+-N and ureide-N in the nodules when GS and GOGAT activities decreased. Therefore, the results provide evidence that N2 fixation in common bean submitted to heat stress is limited by NH4+ assimilation via GS-GOGAT rather than by decreased activity of nitrogenase. Rhizobial effectiveness determined the degree of down-regulation of GS-GOGAT activity in nodule tissues.
653 $aFixação biológica de nitrogênio
700 1 $aKASCHUK, G.
773 $tEnvironmental and Experimental Botany$gv. 98, p. 32-39, Feb. 2014.
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Embrapa Soja (CNPSO) |
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| Registros recuperados : 40 | |
| 4. |  | KASCHUK, G.; HUNGRIA, M.; ANDRADE, D. S. Diversidade de rizóbios que nodulam o feijoeiro (phaseolus vulgaris L.) sob os sistemas de plantios direto e convencional em Ponta grossa, PR. In: WORLD CONGRESS ON CONSERVATION AGRICULTURE = CONGRESSO MUNDIAL SOBRE AGRICULTURA CONSERVACIONISTA, 2., 2003, Foz do Iguaçu. Producing in harmony with nature: [proceedings ...] = Produzindo em harmonia com a natureza: [anais ...]. Ponta Grossa: Federação Brasileira de Plantio Direto na Palha, 2003. v. 2 p. 452-455. II World Congress on Conservation Agriculture, Iguassu Falls, 2003. Extended summary / posters.| Biblioteca(s): Embrapa Soja. |
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| 5. | | KASCHUK, G.; ALBERTON, O.; HUNGRIA, M. Três décadas de estudos sobre biomassa microbiana nos ecossistemas brasileiros: lições aprendidas sobre qualidade do solo e indicadores de sustentabilidade. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa: SBCS, 2010. 4 p. Trab. 1501. 1 CD-ROM. FERTBIO 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | KASCHUK, G.; ALBERTON, O.; HUNGRIA, M. Quantificando os efeitos de diferentes usos agrícolas na biomassa microbiana do solo nos biomas brasileiros. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa: SBCS, 2010. 4 p. Trab. 437. 1 CD-ROM. FERTBIO 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | LOUREIRO, M. de F.; KASCHUK, G.; ALBERTON, O.; HUNGRIA, M. Soybean [Glycine max (L.) Merrill] rhizobial diversity in Brazilian oxisols under various soil, cropping, and inoculation managements. Biology and Fertility of Soils, Berlin, v. 43, n. 6. p. 665-674, Aug. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 12. | | ALBERTONI, O.; ALEIXO, A. P.; URCOVICHE, R. C.; ROSSETO, P.; KASCHUK, G.; HUNGRIA, M. Abundância de fungos micorrízicos arbusculares e biomassa total fúngica e bacteriana de solos cultivados com cana de açúcar. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa: SBCS, 2012. 4 p. Trab. 108. 1 CD-ROM. Fertbio.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | KASCHUK, G.; KUYPER, T. W.; LEFFELAAR, P. A.; HUNGRIA, M.; GILLER, K. E. Are the rates of photosynthesis stimulated by the carbon sink strength of rhizobial and arbuscular mycorrhizal symbioses? Soil Biology & Biochemistry, Oxford, v. 41, n. 6, p. 1233-1244, june 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 40 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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